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| Registros recuperados: 273 | |
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| Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel. |
| Background In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf |
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| David, Elise; Boudry, Pierre; Degremont, Lionel; Tanguy, Amelie; Quere, N; Samain, Jean-francois; Moraga, D. |
| Large-scale mortality events have been observed in Pacific oyster Crassostrea gigas on the west coast of France since the early 1980s, particularly during summer. In order to understand the causes of this mortality, two generations of oysters from single-pair matings were studied in three sites on the French Atlantic coast (Baie-des-Veys, Auray and Ronce-les-Bains). The present paper examines the role of two candidate genes in the susceptibility of oysters to summer mortality, and the selective pressure exerted by such mortality on their polymorphism. Glutamine synthetase (amino-acid metabolism) and delta-9 desaturase (lipid metabolism) genes were studied in the successive generations, using polymerase chain reaction single-strand conformation polymorphism... |
| Tipo: Text |
Palavras-chave: SSCP; Mortality; Glutamine synthetase; Delta 9 desaturase; Crassostrea gigas. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3040.pdf |
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| Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre. |
| In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat... |
| Tipo: Text |
Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf |
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| Morvezen, Romain; Charrier, Gregory; Boudry, Pierre; Chauvaud, Laurent; Breton, Florian; Strand, Oivind; Laroche, Jean. |
| The great scallop Pecten maximus is harvested in several European countries and fisheries targeting this species are severely regulated by fishing quotas. In addition, hatchery-based population enhancement has been developed in some countries to provide alternative or complementary production. The genetic structure of wild populations of P. maximus and the potential impact of aquaculture on the genetic diversity of this species remains poorly documented. In this study, we explored the genetic structure of P. maximus using 12 microsatellite markers, considering 14 populations sampled from Galicia (Spain) to the North of Norway, and one population of Pecten jacobaeus (L., 1758) from the Lion Gulf (Mediterranean Sea). Results indicated a clear differentiation... |
| Tipo: Text |
Palavras-chave: Great scallop; Pecten maximus; Microsatellites; Population genetics; Aquaculture. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00311/42251/41692.pdf |
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| Meistertzheim, Anne-leila; Arnaud-haond, Sophie; Boudry, Pierre; Thebault, Marie-therese. |
| As a result of aquaculture activities, Pacific oysters Crassostrea gigas (Thunberg, 1793) have invaded European coasts. Using seven microsatellites, we found virtually no genetic differentiation between natural populations throughout the European range (from the south of the Wadden Sea (the Netherlands) to the south of France) and French cultivated oysters. The genetic homogeneity of Pacific oyster samples appears to be the result of repeated transfers from same seed stocks made for aquaculture and, to a lesser extent, widespread dispersal due to specific biological traits of this species. The only genetic differentiation of Sylt population in the north of the Wadden Sea (Germany) suggests a stronger, persistent impact of ongoing supply of new genetic... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00119/23051/20924.pdf |
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| Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno. |
| The most significant genetic improvement for production of Pacific oyster (Crassastrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have slowed the development of selective breeding programs. Recently, in the USA, Australia and New Zealand, family-based selective breeding programs have been initiated to improve growth and yield. In Europe, where both natural and... |
| Tipo: Text |
Palavras-chave: Microsatellites; Quantitative genetic; Genetic variability; Crassostrea gigas; Oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2861.pdf |
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| Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno. |
| To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective... |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf |
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| Boudry, Pierre; Degremont, Lionel; Bedier, Edouard; Pouvreau, Stephane; Normand, Julien; Ernande, Bruno. |
| To date, the most significant method to genetically reduce reproductive effort in the Pacific oyster (Crassostrea gigas) has been through the production of triploids, especially since the development of tetraploid lines allowing the breeding of 'natural' triploids. Gametogenesis of triploid oysters is strongly reduced compared with diploids, although they are not fully sterile and can produce viable gametes and some progenies when crossed with diploids. Reduced reproductive allocation and higher heterozygocity are commonly proposed as the main reasons why triploids often present superior yield compared with diploids. Temperature and food availability are known to favour gametogenesis in triploids, but the existence of genetic variation for this trait... |
| Tipo: Text |
Palavras-chave: Selective breeding; Reproduction; Genetic variation; Crassostrea gigas; Pacific oyster; Genetic. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3445.pdf |
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| Ernande, Bruno; Haure, Joel; Degremont, Lionel; Bedier, Edouard; Boudry, Pierre. |
| Phenotypic and genetic correlations between fitness-related life-history traits - such as survival, growth and reproductive allocation - have to be considered to better understand selective processes, both in the wild and in breeding programs. Quantitative genetic experiments, based on nested half-sib mating designs, were perfomed in the Pacific oyster C. gigas (1) to estimate genetic variance and the response to selection and (2) to document phenotypic and genetic trade-offs between life history traits. Physiological trade-offs, i.e. plastcity of resource allocation, were shown to vary among genotypes. Genetic polymorphism was primarily observed for the the plasticity of reproductive effort. In addition, the degree of plasticity in reproductive effort... |
| Tipo: Text |
Palavras-chave: Genetic polymorphism; Genetic; Plasticity; Genetical basis; Crassostrea gigas; Pacific oysters. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-3443.pdf |
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| Boudry, Pierre; Chatain, Beatrice; Naciri-graven, Yamama; Lemaire, Christophe; Gerard, Andre. |
| In France, marine fish and shellfish farming is based mainly on the breeding of wild species, whose natural populations are traditionally exploited. Research programs are conducted by IFREMER, in co-operation with INRA and Universities. French research programs for the genetical improvement of marine fish and shellfish concern primarily seabass (Dicentrarchus labrax) and oysters (Ostrea edulis and Crassostrea gigas). The following programs are developed: The knowledge of wild and farmed populations. The most recent prodjects involve the highly variable microsatellite markers. Seven markers are presently available for seabass and the work is now initiated on turbot (Scophthalmus maximus). In molluscs, microsatellite markers have been developed for the... |
| Tipo: Text |
Palavras-chave: Selection; Triploid; Shellfish; Fish; Genetic. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/1996/acte-3206.pdf |
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| Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre. |
| Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials... |
| Tipo: Text |
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Cornette, Florence; Lapegue, Sylvie. |
| Originaire du nord de l’Asie, l'huître creuse, Crassostrea gigas, a été introduite et transférée, principalement pour l’aquaculture, dans plusieurs pays européens depuis le début des années 1970. Le caractère invasif de cette espèce a été observé au cours de la dernière décennie dans plusieurs pays et elle a même été considérée comme une espèce nuisible dans certaines régions, telles que la mer de Wadden. Dans le cadre du projet HiFlo, soutenu par l’ANR, nous avons pour objectif d'identifier les caractéristiques d'une telle espèce florissante: son succès peut-il être expliqué par le hasard des opportunités (expansion de leur niche écologique) ou est-il lié à des processus évolutifs, ce qui impliquerait une adaptation génétique de l'espèce à de nouvelles... |
| Tipo: Text |
Palavras-chave: Huîtres; Crassostrea gigas; Génétique; Génétique des populations. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00014/12552/9418.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3). Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Invasive; AFLP; Genome scan; Crassostrea gigas. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4542.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre. |
| Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different... |
| Tipo: Text |
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF |
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| Riviere, Guillaume; Klopp, Christophe; Ibouniyamine, Nabihoudine; Huvet, Arnaud; Boudry, Pierre; Favrel, Pascal. |
| Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly... |
| Tipo: Text |
Palavras-chave: Oyster; Transcriptome; Development; Stress; Tissues; NGS. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00302/41321/40520.pdf |
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| Cannuel, Rozenn; Beninger, Peter G.; Mccombie, Helen; Boudry, Pierre. |
| Study of gill development in bivalve larvae and postlarvae provides information on the evolution of this organ and feeding mechanisms of early stages. Scanning electron microscopy was used to document the development of the filibranch homorhabdic gill in hatchery-reared larval, postlarval, and juvenile Mytilus edulis. Four key stages were identified during gill development: (1) transfer of the particle collection function from velum to gill at metamorphosis, with subsequent elongation of the gill filaments to form a gill basket, with complete frontal ciliation; (2) reflection of the inner demibranchs, and transition to a V-shaped gill; (3) delayed development of the outer demibranchs, occuring simultaneously along the gill axis, with transition to the... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6935.pdf |
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| Lambert, Christophe; Soudant, Phillippe; Degremont, Lionel; Delaporte, Maryse; Moal, Jeanne; Boudry, Pierre; Jean, Frédéric; Huvet, Arnaud; Samain, Jean-francois. |
| High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Riviere d'Auray (Brittany) and Ronce (Marennes-oleron Basin, Poitou Charentes). Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered... |
| Tipo: Text |
Palavras-chave: Genetic; Flow cytometry; Reactive oxygen species ROS; Adhesion; Hemocyte; Crassostrea gigas; Summer mortality. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3054.pdf |
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| Avia, Komlan; Coelho, Susana M.; Montecinos, Gabriel J.; Cormier, Alexandre; Lerck, Fiona; Mauger, Stephane; Faugeron, Sylvain; Valero, Myriam; Cock, J. Mark; Boudry, Pierre. |
| Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48779/49186.pdf |
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| Barre, Marc; Naciri, Yamama; Boudry, Pierre; Goyard, Emmanuel; Launey, Sophie; Cochennec, Nathalie; Heurtebise, Serge; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre. |
| The Aquaculture and Pathological Genetics (GAP) laboratory at La Tremblade has been monitoring three populations of European flat oyster since 1985 or 1989 under selection for higher survival rates in the presence of the parasite Bonamia ostrea. One selection cycle (1985 population) or two (1989 populations) were carried out by mass selection by placing the oysters for several years in a highly infested zone, and then harvesting spawns from survivors. |
| Tipo: Text |
Palavras-chave: Huîtres plates; Génétique; Selection génétique; Ostrea edulis. |
| Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/acte-3244.pdf |
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| Huvet, Arnaud; Fabioux, Caroline; Lapegue, Sylvie; Boudry, Pierre. |
| Crassostrea angulata and Crassostrea gigas, two cupped oysters of Asian origin (O'Foighil et al, 1998), have been introduced successively in Europe during the XVlth and the XXth century (Grizel et Héral, 1991). The present populations of C. angulata appear to be limited to the south of Spain and Portugal, whereas the distribution of C. gigas in Europe is rising because of the development of oyster farming. The distinction between the two subspecies in based on a RFLP marker of the mitochondrial genome (COI) which shows a strong genetic differentiation between the two subspecies populations (Boudry et al., 1998). On the contrary, the nuclear markers (microsatellites) showed a smaller genetic differentiation between them (Huvet et al, under press). The... |
| Tipo: Text |
Palavras-chave: Microsatellite; Hybridation; Crassostrea angulata; Crassostrea gigas; Huîtres; Génétique. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3282.pdf |
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| Registros recuperados: 273 | |
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